AIL – Microscopes

 

Leica SP5 inverted

Objectives

  • 20x/0.70 HC PL APO DIC
  • 40x/1.25 CS HCX PL APO DIC Oil
  • 63x/1.40 HCX PL APO DIC Oil
  • 100x/1.40 HCX PL APO DIC Oil

Laser lines

  • 405nm, 488nm, 543nm, 633nm

Detectors

  • 3 spectral PMTs
  • 1 transmitted light PMT

Other features

  • Resonant scanner

Zeiss LSM510 upright

Objectives

  • 5x EC Plan-Neofluar
  • 10x EC Plan-Neofluar Ph
  • 20x EC Plan-Neofluar
  • 40x EC Plan-Neofluar Ph
  • 40x EC Plan-Neofluar DIC Oil
  • 63x Plan Apochromat DIC Oil

Laser lines

  • 405nm, 458/477/488/514nm, 543nm, 633nm

Detectors

  • 3 filter based PMTs
  • 1 transmitted light PMT

Olympus IX81 inverted

Objectives

  • 10x/0.30 UPlan FLN Ph
  • 20x/0.45 LUCPlan FLN Ph
  • 40x/0.60 LUCPlan FLN DIC
  • 60x/0.70 LUCPlan FLN DIC

Filters

  • DAPI, FITC, Texas Red, Cy5

Camera

  • Photometrics CoolSNAP Myo

Other features

  • Motorised stage with stage-top incubator

Zeiss AxioImager Z1 upright

Objectives

  • 2.5x/0.06 EC Plan-Neofluar
  • 5x/0.13 EC Plan-Neofluar
  • 10x/0.30 EC Plan-Neofluar
  • 20x/0.50 EC Plan-Neofluar
  • 40x/0.75 EC Plan-Neofluar
  • 63x/1.25 EC Plan-Neofluar Oil
  • 100x/1.30 EC Plan-Neofluar Oil

Filters

  • DAPI, GFP, Rhodamine, Cy5

Camera

  • Axiocam HRM B&W

Olympus FV3000RS inverted confocal

Objectives

  • 1.25x/0.04 PL APO
  • 10x/0.4 UPL SAPO DIC
  • 20x/0.75 UPL SAPO DIC
  • 40x/0.95 UPL SAPO DIC
  • 60x/1.35 UPL APO DIC Oil
  • 100x/1.35 UPL SAPO DIC Silicone

Laser lines

405nm, 445nm, 488nm, 514nm, 561nm, 640nm

Detectors

  • 2 spectral PMTs
  • 2 high sensitivity spectral GaAsp detectors
  • 1 transmitted light PMT

Other features

  • Resonant scanner
  • Motorised stage with stage-top incubator
  • Super-resolution software module
  • 3D Deconvolution software module

Zeiss PALM – LCM laser microdissection

Objectives

  • 5x/0.25 Fluar
  • 10x/0.50 Fluar
  • 20x/0.40 LD Plan-Neofluar
  • 40x/0.6 LD Plan-Neofluar
  • 63x/0.75 LD Plan-Neofluar

Filters

DAPI, GFP, Rhodamine, Cy5

Cameras

  • Axiocam MRM B&W
  • Axiocam ICC1 colour