Electron Microscopy Laboratory

Exploring ultrastructure and beyond

Electron Microscopy is a powerful imaging tool for analysis of composition, ultrastructure and function of biological samples and materials. From tissues and cells to subcellular structures and macromolecular complexes, Transmission and Scanning electron microscopy provides 2D and 3D morphological analysis with optional detection and localization of specific antigens by immuno-gold labelling.

Technology, Equipment and Expertise

  • Samples processing (RT & Cryo)
  • Ultrathin- sectioning & cryo-sectioning
  • Antigen detection by immuno-gold EM
  • Ultrastructure analysis by (cryo-) EM
  • 3D Electron tomography
  • Correlative light and electron microscopy
  • Image analysis and 3D cellular modelling

Equipment available

  • FEI Titan Krios &JEOL 2200FS cryo-TEM
  • FEI Tecnai T12 & Spirit G2 Icorr TEM
  • FEI Quanta 650 & JEOL JSM 6701F FEG SEM

The Electron Microscopy Lab provides advanced expertise in electron microscopy: from sample preparation to image analysis and for a large variety of biological samples ranging from macromolecules to tissues. Services provided include consultation on experiment design and sample preparation, sample processing, image acquisition and data analysis.

To enquire more about Electron Microscopy Laboratory, please contact Dr Isabelle Bonne at lsigebim@nus.edu.sg .